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Density Gradient Media

Density Gradient Media – OptiPrep, NycoPrep, LymphoPrep, PolymorphPrep, Nycodenz

PROGEN Biotechnik offers a range of iodinated density gradient media developed by Axis Shield which are in compliance with FDA and EU cGMP standards and regulatory requirements: OptiPrep, NycoPrep, LymphoPrep, PolymorphPrep, Nycodenz.

Much of modern cell and molecular biology relies on the isolation of pure, functionally intact populations of cells, subcellular organelles and macromolecules in high yield and both research and clinical applications provide an impetus for the development of improved separation technology. The media are used for the purification not only of cells and organelles but also membrane vesicles, viruses, proteins, nucleic acids and lipoproteins.

The density gradient media are

  • proven to be non-toxic,
  • have a low osmolality and
  • are sufficiently dense to permit the purification of all types of biological particles.

PROGEN Biotechnik GmbH offers the following Density Gradient Media for separating and fractionating of cells and cellular organelles

OptiPrep™ is a ready-made, sterile and endotoxin-tested solution of iodixanol. Suitable for the isolation of many different cell types from blood and tissue (e.g. monocytes, lymphocytes, granulocytes, platelets, dendritic cells, megakaryotic progenitor cells; liver non-parenchymal cells, islet of Langerhans cells, spermatozoa; plant protoplasts) as well as for the isolation of cell organelles (e.g. nuclei, mitochondria, peroxisomes, lysosomes, endosomal subfractions) and membrane fractions (e.g. Golgi membranes, endoplasmic reticulum, specific plasma membrane domains). Separation of viable from non-viable cells. Also suitable for self-generated gradients. Highly effective for purification of viruses and virus particles. Also suitable for isolation of proteins, plasma lipoproteins and plasmid DNA, fractionation of ribonucleoproteins.


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NycoPrep™ 1.068 is a specially formulated sterile and endotoxin-tested medium devised by Boyum to separate human monocytes.


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Nycoprep™ 1.077 is a ready-made, sterile and endotoxin tested solution for the purification of human mononuclear cells.


More Information about NycoPrep™ 1.077 (4x250mL)

Nycoprep™ 1.077 is a ready-made, sterile and endotoxin tested solution for the purification of human mononuclear cells.


More Information about NycoPrep™ 1.077 (1x250mL)

LymphoPrep™ Tube is a sterile tube in which the LymphoPrep™ is contained below a plastic filter disc. Especially useful for the separation of monocytes (monocytes and lymphocytes) from whole blood.


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LymphoPrep™ is a ready-made, sterile and endotoxin tested solution suitable for the purification of human mononuclear cells (monocytes and lymphocytes).


More Information about LymphoPrep™ (1x250mL)

LymphoPrep™ is a ready-made, sterile and endotoxin tested solution suitable for the purification of human mononuclear cells (monocytes and lymphocytes).


More Information about LymphoPrep™ (4x250mL)

LymphoPrep™ is a ready-made, sterile and endotoxin tested solution suitable for the purification of human mononuclear cells (monocytes and lymphocytes).


More Information about LymphoPrep™ (6x500mL)

PolymorphPrep™ is a ready-made, sterile and endotoxin tested solution for the isolation of human polymorphonuclear leucocytes (granulocytes) from whole blood.


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Freely soluble in water up to 80% (w/v). Suitable for the the isolation of many different cell types from blood and tissue (e.g. monocytes, lymphocytes, granulocytes, platelets, dendritic cells, megakaryotic progenitor cells; liver non-parenchymal cells, islet of Langerhans cells, spermatozoa; plant protoplasts) as well as for the isolation of cell organelles (e.g. nuclei, mitochondria, peroxisomes, lysosomes, endosomal subfractions) and membrane fractions (e.g. Golgi membranes, endoplasmic reticulum, specific plasma membrane domains). Separation of viable from non-viable cells. Also suitable for self-generated gradients. Highly effective for purification of viruses and virus particles. Also suitable for isolation of proteins, plasma lipoproteins and plasmid DNA, fractionation of ribonucleoproteins.


More Information about Nycodenz®